QEL Chennai

Form No. 75 Quality Evaluation Laboratory, Spices Board, Chennai

Details of the analysis on various parameters showing the analytical charges, minimum quantity and number of days required for the completion of analysis.

Sl No. Name of Analysis Min. Qty Reqd in Gm Charges1
(Rs) No. Of Days Required Physico- Chemical Analysis 1 Acid Insoluble Ash 250 450 3 2 Added Starch 100 350 2 3 Alcohol Soluble Extract 250 300 3 4 Bulk Density/ Litre Weight of Spices 1000 300 1 5 Capsaicin –HPLC Method(%/SHU) 100 1800 4 6 Calcium as Calcium Oxide 250 350 5 7 Coumarin Content 100 1800 3 8 Chromate Test( Qualitative) 100 350 2 9 Cold Water Soluble Extract 100 350 4 10 Color Value (ASTA Method) 100 500 3 11 Common salt 100 450 3 12 Crocine 10 1000 2 13 Crude Fiber 100 700 5 14 Curcumin (ASTA Method) 100 600 3 15 Excreta, Mammalian 500/250 350 1 16 Excreta, Others 500/250 350 1 17 Extraneous/Foreign Matter 500/250 350 1 18 Filth, Heavy 100 600 2 19 Filth, Light 100# 600 5 20 Gingerols & Shogaols 100 2400 3 21 Insect Defiled/ Infested 500/250 350 1 22 Light Berries
250 350 1 23 Moisture (ASTA Method) 250 450 2 24 Mould( Physical) 250 350 1 25 Non-Volatile Ether Extract 100 550 6 26 Oleoresin(EDC Extractables) 100 500 5 27 Other Seeds
100 350 1 28 Other Seeds in Cumin 100 350 1 29 Picrocrocine 10 1000 2 30 Piperine (ASTA Method) 100 1000 3 31 Safranal 10 850 2 32 Starch 100 850 5 33 Total Ash 100 450 3 34

1 28 Other Seeds in Cumin 100 350 1 29 Picrocrocine 10 1000 2 30 Piperine (ASTA Method) 100 1000 3 31 Safranal 10 850 2 32 Starch 100 850 5 33 Total Ash 100 450 3 34 Volatile Oil 200 450 3 35 Vanillin 30 1800 3 36 Vanillic Acid 30 1800 3

Quality Evaluation Laboratory, Spices Board, Chennai

Details of the analysis on various parameters showing the analytical charges, minimum quantity and number of days required for the completion of analysis.

Sl No. Name of Analysis Min. Qty Reqd (g) Charges in Rs. No. Of Days Required 37 P-hydroxybenzoic Acid 30 1800 3 38 P-hydroxybenzaldehyde 30 1800 3 39 Water soluble Ash 100 550 4 40 whole Insects, Dead (By Count) 250 350 1 Residual Analysis 41 Aflatoxin(B1, B2, G1 & G2) (HPLC-Method) 250 2700 3 42 Heavy Metals (Cd, Cr, Cu, Fe, Mg, Mo, Pb & Zn) 100 500## 5 43 Mineral Oil in Black Pepper 500 600 2 44 Ochratoxin A(HPLC-Method) 250 3000 3 45 Organophosphorous Pesticides Residues-(I) (Chlorpyriphos, Dimethoate, Disulfoton,Ethion, Methylparathion,Phorate,Parathion &Quinalphos) 200 3000 6 46 *Pesticide Residues-III (Iprobenphos, Triazophos & Profenphos) 200 3000 6 47 Organophosphorous Pesticides Residues-(II) (Methylpirimiphos, Phosalone & Monocrotophos) 200 3000 6 48 *Pesticide Residues-IV (Ethion, Iprobenphos,Triazophos& Profenphos) 200 4800 6 49 *Pesticide Residues-V (Profenphos, Bifenthrin, Triazophos, Diethion & Endosulfan) 500 6000 6 50 Pesticide Residue-Malathion 100 1800 6 51

enphos,Triazophos& Profenphos) 200 4800 6 49 Pesticide Residues-V (Profenphos, Bifenthrin, Triazophos, Diethion & Endosulfan) 500 6000 6 50 Pesticide Residue-Malathion 100 1800 6 51 Pesticide Residue- Phorate 100 1800 6 52 Pesticide Residue- Etrimphos 100 1800 6 53 Pesticide Residue- Fenitrothion 100 1800 6 54 Pesticide Residue- Phosmet 100 1800 6 55 Pesticide Residue- Chlorfenvinphos 100 1800 6 56 Pesticide Residue- Parathion 100 1800 6 57 Pesticide Residues-VI (Ethion, Iprobenfos, Triazophos, Profenofos, Phorate, Parathion, Chlorpyrifos & Methylparathion) 300 7000 6 58 Pesticide Residues. Single Compound( any of the above) 200 1800 6 59 Sudan – Dye( I,II,III & IV(HPLC Method)) 100 2400 2 60 Sudan – Dye( I,II,III & IV(LC-MS/MS Method)) 100 3000 2 61 Synthetic Dyes II( Sudan Red 7b, Sudan orange G, Parared, Rhodamine B & Butter yellow (LC-MS/MS Method) 100 3000 2 62 Synthetic Dyes II-Single compound (Any of the above) 100 1800 2 63 Sunset Yellow FCF in Sugar Coated Fennel 100 1800 2 Microbiological Analysis 64 Aerobic Plate Count 100 600 3 65 Aerobic Spore Count 100 600 4 66 Bacillus cereus 100 1000 5

2 63 Sunset Yellow FCF in Sugar Coated Fennel 100 1800 2 Microbiological Analysis 64 Aerobic Plate Count 100 600 3 65 Aerobic Spore Count 100 600 4 66 Bacillus cereus 100 1000 5

Quality Evaluation Laboratory, Spices Board, Chennai

Details of the analysis on various parameters showing the analytical charges, minimum quantity and number of days required for the completion of analysis.

Sl No. Name of Analysis Min. Qty Reqd in Gm Charges in Rs. No. Of Days Required 67 Bacillus cereus ( TEMPO Method) 100 1200 2 68 Coliforms 100 550 5 69 Coliforms ( TEMPO Method) 100 900 2 70 Enterobacteriaceae 100 600 5 71 Enterobacteriaceae (TEMPO Method) 100 1000 2 72 E. coli 100 900 8 73 E.coli (TEMPO Method) 100 900 2 74 Mould 100 500 6 75 Salmonella ( FDA BAM) 375 1700 8 76 Salmonella ( AOAC OMA Method) 375 1700 3 77 Staphylococcus aureus 100 900 5 78 Staphylococcus aureus (TEMPO Method) 100 1000 2 79 Thermostable Bacteria 100 600 3 80 Total Viable Count (TEMPO Method) 100 900 2 81 Yeast 100 500 6 82 Yeast & Mould 100 500 6 83 Yeast & Mould (TEMPO Method) 100 1200 3 NOTE:-

Analytical charges revised with effect from 01-08-2017

1. Service Tax @ 18.0% will be charge extra as per govt. norms. 2.* Charges based on the parameter specified for each spice. 3.** Turmeric is analyzed for Sudan I - IV by HPLC Method unless otherwise specified 4.# Quantity for light filth analysis for tamarind concentrate by AOAC Method should be a minimum of 600 g

. 3.** Turmeric is analyzed for Sudan I - IV by HPLC Method unless otherwise specified 4.# Quantity for light filth analysis for tamarind concentrate by AOAC Method should be a minimum of 600 g 5. ## Analytical charges Rs.500 /- per element. 6. In the case of analysis of oleoresin samples of spice of the above parameters a minimum of 50 g sample is required & in case of coriander and herbs, a sample size of 300 g is required. 7. The days required for the analysis will increase depending on the inflow of sample for analysis. 8.The samples may be send to the Deputy Director, Regional Office ,Spices Board, Plot No R-11, North end Road, Sipcot Industrial Complex, Gummidipoondi, Tamil Nadu ,601201 along with analytical charges in the form of D.D . drawn in favor of Secretary, Spices Board. Kochi-25